Other equipment

Super-resolution microscope for live cell monitoring ZEISS ELYRA 7 Lattice SIM

The Zeiss Elyra 7 system is based on the Axio Observer 7 (inverted stand) equipped with an incubator for live-cell imaging with temperature and CO₂ control. The microscope is equipped with two cooled sCMOS cameras for simultaneous two-color and sequential four-color (4-channel) imaging. The Zeiss Elyra 7 is a wide-field based super-resolution system that uses a specialized 3D Lattice illumination pattern for 3D structured-illumination (SIM) images. The lattice illumination enables faster, gentler, and deeper imaging of living specimens at high speed in Burst Mode. The SIM² processing mode can double the resolution of the regular SIM, producing images with an effective resolution down to ~60 nm in x and y and ~200nm in z. The Zeiss Elyra 7 system also enables total internal reflection fluorescence microscopy (TIRFM) and single-molecule localization microscopy (SMLM).

Available are:

Objectives:

EC Plan-Neofluar 10x/0.3 M27 (FWD=5.2mm)
Plan-Apochromat 20x/0.8 M27 (FWD=0.55mm)
Plan-Apochromat 40x/1.4 Oil DIC M27 (FWD=0.13mm), (UV)VIS-IR
Plan-Apochromat 63x/1.4 Oil DIC M27 (FWD=0.19mm)
Plan-Apochromat 63x/1.46 Oil Corr M27 TIRF (FWD=0.10mm)

Filters:

Filter set FLEX min is equipped with dual and triple emission bands and allows for fast sequential two channel acquisition by fast switching of the laser lines.
MBS (major beam splitter) 405/488/561/641 and EF (emission filter) LBF 405/488/561/641
MBS 405/488/561/641 and EF BP 420-480 / BP 495-525 / LP 650

Motorised System for Dynamic Fluorescence Microscopy Leica Thunder

Thunder Imager Live Cell system consists of fully motorized inverted fluorescent microscope DMi8 (Leica) with adaptive focus control for drift correction and fast focusing, deep-cooled 4.2 MP sCMOS Leica DFC9000 Gt fast camera, black incubator i8 BLACK LS (PeCon) with optional CO₂ and temperature control. Images are acquired with the software LAS X. As a light source, a system of 8 LED diodes, the LED8 (395, 438, 475, 511, 555, 575, 635, 730 nm) and UV light source the CoolLED pE340^fura (CoolLED) are available.

Available are:

Objectives:
- N-plan 5x/0.12 PH 0 (No. 11506303, Leica)
- HC PL FLUOTAR 10x/0.32 PH 1 (No. 11506522, Leica)
- HC PL FLUOTAR 20x/0.55 PH 2 (No. 11506518, Leica)
- HC PL FLUOTAR L 40x/0.60 Corr. (No. 11506201, Leica)
- HC PL FL 63x/0.90 Corr. (No. 11506223, Leica)
- HC FLUOTAR (340) 100x/1.30, oil (No. 11506368, Leica)
Filters:
- Filter cube DAPI (excitation: 350/50, dichroic: 400, emission: 460/50)
- Filter cube CYR71010 for use with multi-LED light sources (excitation: 436/28, 506/21, 578/24, 730/40; dichroic: 459, 523, 598, 763, emission: 473/22, 539/24, 641/78, 810/80)
- Filter cube DFT51010 for use with multi-LED light sources (excitation: 391/32, 479/33, 554/24, 638/31, dichroic: 415, 500, 572, 660, emission: 435/30, 519/25, 594/32, 695/58)
- Filter wheel LED8 with five filters (460/80, 535/79, 590/50, 642/80, 100%)