Other equipment

Thunder Imager Live Cell system consists of fully motorized inverted fluorescent microscope DMi8 (Leica) with adaptive focus control for drift correction and fast focusing, deep-cooled 4.2 MP sCMOS Leica DFC9000 Gt fast camera, black incubator i8 BLACK LS (PeCon) with optional CO₂ and temperature control. Images are acquired with the software LAS X. As a light source, a system of 8 LED diodes, the LED8 (395, 438, 475, 511, 555, 575, 635, 730 nm) and UV light source the CoolLED pE340^fura (CoolLED) are available.

Available are:

• Objectives:
  • N-plan 5x/0.12 PH 0 (No. 11506303, Leica)
  • HC PL FLUOTAR 10x/0.32 PH 1 (No. 11506522, Leica)
  • HC PL FLUOTAR 20x/0.55 PH 2 (No. 11506518, Leica)
  • HC PL FLUOTAR L 40x/0.60 Corr. (No. 11506201, Leica)
  • HC PL FL 63x/0.90 Corr. (No. 11506223, Leica)
  • HC FLUOTAR (340) 100x/1.30, oil (No. 11506368, Leica)
• Filters:
  • Filter cube DAPI (excitation: 350/50, dichroic: 400, emission: 460/50)
  • Filter cube CYR71010 for use with multi-LED light sources (excitation: 436/28, 506/21, 578/24, 730/40; dichroic: 459, 523, 598, 763, emission: 473/22, 539/24, 641/78, 810/80)
  • Filter cube DFT51010 for use with multi-LED light sources (excitation: 391/32, 479/33, 554/24, 638/31, dichroic: 415, 500, 572, 660, emission: 435/30, 519/25, 594/32, 695/58)
  • Filter wheel LED8 with five filters (460/80, 535/79, 590/50, 642/80, 100%)​

It consists of Axiovert 200 (Zeiss) fluorescent inverted microscope with digital sCMOS camera Prime 4.2 (Photometrics) and CO2 chamber. It has VisiView (Visitron Systems) image acquisition software and tuneable light source High Speed Polychromatic Illumination system (VisiChrome).

Available are:

• Objectives:
  • A-plan 10x/0.25
  • LD Achroplan 20x/0.40 Corr
  • LD Achroplan 40x/0.60 Corr
  • EC Plan-Neofluar 63x/0.95 Corr
  • Plan-Apochromat 63x/1.4 Oil
  • Plan-Apochromat 100x/1.4 Oil Iris
• Filters:
  • Anneps- Chroma 71006 (Ex. 440nm, 530nm;Em. D605/54m)
  • Fura-Chroma 71000 (Ex. 340nm, 380nm; Em. D510/40m)
  • TOTO-Chroma 41028 (Ex. 514nm; Em. D535/30m)
  • DAPI- Chroma 31000 (Ex. 360nm; Em. D510/40m)
  • TMRE-Chroma 31003 (Ex. 549nm; Em. D580/30m)
  • GFP-Chroma 31019 (Ex. 425nm; Em. D505/40m)

Attune NxT (Life technologies).

Available are:

• Excitation lasers:
  • Blue (488 nm)
  • Violet (405 nm)
  • Red (637 nm)
• Autosampler for 96 and 384 well plate measurements