Thunder Imager Live Cell system consists of fully motorized inverted fluorescent microscope DMi8 (Leica) with adaptive focus control for drift correction and fast focusing, deep-cooled 4.2 MP sCMOS Leica DFC9000 Gt fast camera, black incubator i8 BLACK LS (PeCon) with optional CO2 and temperature control. Images are acquired with the software LAS X. As a light source, a system of 8 LED diodes, the LED8 (395, 438, 475, 511, 555, 575, 635, 730 nm) and UV light source the CoolLED pE340fura (CoolLED) are available.

Available are:

  • Objectives:
    • N-plan 5x/0.12 PH 0 (No. 11506303, Leica)
    • HC PL FLUOTAR 10x/0.32 PH 1 (No. 11506522, Leica)
    • HC PL FLUOTAR 20x/0.55 PH 2 (No. 11506518, Leica)
    • HC PL FLUOTAR L 40x/0.60 Corr. (No. 11506201, Leica)
    • HC PL FL 63x/0.90 Corr. (No. 11506223, Leica)
    • HC FLUOTAR (340) 100x/1.30, oil (No. 11506368, Leica)
  • Filters:
    • Filter cube DAPI (excitation: 350/50, dichroic: 400, emission: 460/50)
    • Filter cube CYR71010 for use with multi-LED light sources (excitation: 436/28, 506/21, 578/24, 730/40; dichroic: 459, 523, 598, 763, emission: 473/22, 539/24, 641/78, 810/80)
    • Filter cube DFT51010 for use with multi-LED light sources (excitation: 391/32, 479/33, 554/24, 638/31, dichroic: 415, 500, 572, 660, emission: 435/30, 519/25, 594/32, 695/58)
    • Filter wheel LED8 with five filters (460/80, 535/79, 590/50, 642/80, 100%)​