The Zeiss Elyra 7 system is based on the Axio Observer 7 (inverted stand) equipped with an incubator for live-cell imaging with temperature and CO2 control. The microscope is equipped with two cooled sCMOS cameras for simultaneous two-color and sequential four-color (4-channel) imaging. The Zeiss Elyra 7 is a wide-field based super-resolution system that uses a specialized 3D Lattice illumination pattern for 3D structured-illumination (SIM) images. The lattice illumination enables faster, gentler, and deeper imaging of living specimens at high speed in Burst Mode. The SIM² processing mode can double the resolution of the regular SIM, producing images with an effective resolution down to ~60 nm in x and y and ~200nm in z. The Zeiss Elyra 7 system also enables total internal reflection fluorescence microscopy (TIRFM) and single-molecule localization microscopy (SMLM).
Available are:
Objectives:
- EC Plan-Neofluar 10x/0.3 M27 (FWD=5.2mm)
- Plan-Apochromat 20x/0.8 M27 (FWD=0.55mm)
- Plan-Apochromat 40x/1.4 Oil DIC M27 (FWD=0.13mm), (UV)VIS-IR
- Plan-Apochromat 63x/1.4 Oil DIC M27 (FWD=0.19mm)
- Plan-Apochromat 63x/1.46 Oil Corr M27 TIRF (FWD=0.10mm)
Filters:
- Filter set FLEX min is equipped with dual and triple emission bands and allows for fast sequential two channel acquisition by fast switching of the laser lines.
- MBS (major beam splitter) 405/488/561/641 and EF (emission filter) LBF 405/488/561/641
- MBS 405/488/561/641 and EF BP 420-480 / BP 495-525 / LP 650
