Vitae is a pulse generator able to generate square wave high-voltage pulses (amplitudes varying between 500 – 3000 V, pulse length between 50 µs – 1 ms), where applied amplitude and current are measured for each pulse separately. The pulse generator consists of a computer that runs a graphic user interface and user interacts with the device by means of the touch-screen (where probe type, pulse amplitude, length, number and repetition frequency can be selected) incorporated in the display and a double pedal. Vitae pulse generator can also be used for electroporation of smaller cells (i.e. bacteria, yeast) due to its capability to deliver pulses with higher amplitudes.