Gene electro-transfer through the lens of polymer physics (2021-2023) |
| Head: | dr. Shaurya Sachdev, University of Ljubljana, Faculty of Electrical Engineering |
| Partner: | / |
| Funding: | EC – European Commission |
| Code: | 101038051 — GETPolPhys |
The research project is funded by the European Comission.
- Member of University of Ljubljana
- Code
- 101038051 — GETPolPhys
- Project
- Gene electro-transfer through the lens of polymer physics
- Period
- 01.06.2021 - 31.05.2023
- Amount of financing
- 1 FTE
- Head
Shaurya Sachdev, PhD
- Research activity
- ENG - Information Science and Engineering
- Research Organisation
- Abstract
Application of pulsed electric field (PEF) can reversibly increase the permeability of the cell membrane allowing the access of otherwise impermeable DNA molecules to the inside of the cell. Introduction of foreign DNA molecules encoding immunomodulatory proteins, antibodies and antigens into cells using PEF, known as Gene Electro-Transfer (GET), is increasingly used for the modulation of the immune system or immunotherapy. While GET based immunotherapy presents itself as a potent application for treatment of cancer and vaccination against infectious diseases, it is suffering from low levels of transgene expressions in vivo. This low efficiency can largely be attributed to our lack of fundamental understanding of the mechanisms by which DNA molecules overcome the barriers of the extra-cellular matrix and the cell membrane in the presence of an electric field. In this action, I aim to provide this required fundamental understanding using principles of polymer physics, soft matter and statistical mechanics. Experiments based on these principles will be conducted in vitro and in vivo to generate results that can be directly compared to theories and models of DNA transport through the extra-cellular matrix and the cell membrane. Understanding the mechanisms within the frameworks of polymer physics will radically improve the efficiency of GET immunotherapy, because it will provide a mechanistic ground for developing optimum protocols within complex tissue environments that can, at the same time, be readily transferred across tissue types and species.
| Gene electro-transfer through the lens of polymer physics (2021-2023) |
| Head: | dr. Shaurya Sachdev, Univerza v Ljubljani, Fakulteta za elektrotehniko |
| Partner: | / |
| Funding: | EC – European Commission |
| Code: | 101038051 — GETPolPhys |