| Protocol preparation for reserach on reversible and irreversible electroporation of cardiac cells (2023-2023) |
| Head: | Assist. Prof. Lea Rems, University of Ljubljana, Faculty of Electrical Engineering |
| Partner: | / |
| Funding: | Slovenian Research Agency (ARRS), Slovenia |
| Code: | N2-0333 |
The project is funded by the Slovenian Research Agency (ARRS).
- Member of University of Ljubljana
- Code
- N2-0333
- Project
- Protocol preparation for reserach on reversible and irreversible electroporation of cardiac cells
- Period
- 1.10.2023 - 31.12.2023
- Amount of financing
- 0.3 FTE
- Head
- Research activity
- Engineering sciences and technologies - Systems and cybernetics - Biomedical engineering
- Research Organisation
- Abstract
The main objective of the research project ERC Potential is to carry out initial optimisation of the experimental protocols that will be used in our upcoming ERC-StG project. The latter will be focused on electroporation of cardiac cells with the aim of establishing fundamental knowledge needed to develop electroporation-based treatments for cardiac diseases. We will optimise a protocol to monitor the function of primary isolated cardiomyocytes using our new specialised system from IonOptix for simultaneous monitoring of action potentials, intracellular calcium transients, and sarcomere length using optical microscopy. We will optimise the protocol for protein expression analysis using the western blot method. We will establish a protocol for the extraction of collagen from rat tails, which will be used for the preparation of collagen hydrogels in our ERC-StG project. We will carry out the first tests with our new super-resolution microscope Zeiss ELYRA Lattice 7 SIM², which was obtained through the ARIS tender Paket 21 and that will be used to analyse samples in our ERC-StG project. We will also prepare and submit an application for ethical approval to use rats in our ERC-StG project. These preparations will allow for an efficient start of the ERC-StG project.
- Researchers
- The phases of the project and their realization
n/a
- Citations for bibliographic records
List of publications is available from our Publications page.