{"id":769,"date":"2017-08-01T07:00:59","date_gmt":"2017-08-01T07:00:59","guid":{"rendered":"https:\/\/lbk.fe.uni-lj.si\/ic\/?p=769"},"modified":"2024-04-18T07:16:49","modified_gmt":"2024-04-18T07:16:49","slug":"super-resolution-microscope-for-live-cell-monitoring-zeiss-elyra-7-lattice-sim","status":"publish","type":"post","link":"https:\/\/lbk.fe.uni-lj.si\/ic\/en\/equipment\/super-resolution-microscope-for-live-cell-monitoring-zeiss-elyra-7-lattice-sim","title":{"rendered":"Super-resolution microscope for live cell monitoring ZEISS ELYRA 7 Lattice SIM"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: rgba(255,255,255,0);background-position: center center;background-repeat: no-repeat;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;margin-bottom: 0px;margin-top: 0px;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_2_3 2_3 fusion-two-third fusion-column-first\" style=\"width:66.666666666667%;width:calc(66.666666666667% - ( ( 4% ) * 0.66666666666667 ) );margin-right: 4%;margin-top:0px;margin-bottom:20px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-1\"><p>The Zeiss Elyra 7 system is based on the Axio Observer 7 (inverted stand) equipped with an incubator for live-cell imaging with temperature and CO<sub>2<\/sub> control. The microscope is equipped with two cooled sCMOS cameras for simultaneous two-color and sequential four-color (4-channel) imaging. The Zeiss Elyra 7 is a wide-field based super-resolution system that uses a specialized 3D Lattice illumination pattern for 3D structured-illumination (SIM) images. The lattice illumination enables faster, gentler, and deeper imaging of living specimens at high speed in Burst Mode. The SIM\u00b2 processing mode can double the resolution of the regular SIM, producing images with an effective resolution down to ~60 nm in x and y and ~200nm in z. The Zeiss Elyra 7 system also enables total internal reflection fluorescence microscopy (TIRFM) and single-molecule localization microscopy (SMLM).<\/p>\n<p><u>Available are: <\/u><\/p>\n<p>Objectives:<\/p>\n<ul>\n<li>EC Plan-Neofluar 10x\/0.3 M27 (FWD=5.2mm)<\/li>\n<li>Plan-Apochromat 20x\/0.8 M27 (FWD=0.55mm)<\/li>\n<li>Plan-Apochromat 40x\/1.4 Oil DIC M27 (FWD=0.13mm), (UV)VIS-IR<\/li>\n<li>Plan-Apochromat 63x\/1.4 Oil DIC M27 (FWD=0.19mm)<\/li>\n<li>Plan-Apochromat 63x\/1.46 Oil Corr M27 TIRF (FWD=0.10mm)<\/li>\n<\/ul>\n<p>Filters:<\/p>\n<ul>\n<li>Filter set FLEX min is equipped with dual and triple emission bands and allows for fast sequential two channel acquisition by fast switching of the laser lines.<\/li>\n<li>MBS (major beam splitter) 405\/488\/561\/641 and EF (emission filter) LBF 405\/488\/561\/641<\/li>\n<li>MBS 405\/488\/561\/641 and EF BP 420-480 \/ BP 495-525 \/ LP 650<\/li>\n<\/ul>\n<\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_3 1_3 fusion-one-third fusion-column-last\" style=\"width:33.333333333333%;width:calc(33.333333333333% - ( ( 4% ) * 0.33333333333333 ) );margin-top:0px;margin-bottom:20px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><style type=\"text\/css\">.fusion-gallery-1 .fusion-gallery-image {border:0px solid #e2e2e2;}<\/style><div class=\"fusion-gallery fusion-gallery-container fusion-grid-1 fusion-columns-total-2 fusion-gallery-layout-grid fusion-gallery-1\" style=\"margin:-5px;\"><div style=\"padding:5px;\" class=\"fusion-grid-column fusion-gallery-column fusion-gallery-column-1 hover-type-zoomin\"><div class=\"fusion-gallery-image\"><a href=\"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-content\/uploads\/2024\/04\/elyra-7-by-zeiss.png\" rel=\"noreferrer\" data-rel=\"iLightbox[gallery_image_1]\" class=\"fusion-lightbox\" target=\"_self\"><img src=\"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-content\/uploads\/2024\/04\/elyra-7-by-zeiss.png\" width=\"800\" height=\"533\" alt=\"\" title=\"elyra-7-by-zeiss\" aria-label=\"elyra-7-by-zeiss\" class=\"img-responsive wp-image-771\" \/><\/a><\/div><\/div><div class=\"clearfix\"><\/div><div style=\"padding:5px;\" class=\"fusion-grid-column fusion-gallery-column fusion-gallery-column-1 hover-type-zoomin\"><div class=\"fusion-gallery-image\"><a href=\"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-content\/uploads\/2024\/04\/za_web.jpg\" rel=\"noreferrer\" data-rel=\"iLightbox[gallery_image_1]\" class=\"fusion-lightbox\" target=\"_self\"><img src=\"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-content\/uploads\/2024\/04\/za_web.jpg\" width=\"800\" height=\"533\" alt=\"\" title=\"za_web\" aria-label=\"za_web\" class=\"img-responsive wp-image-770\" \/><\/a><\/div><\/div><div class=\"clearfix\"><\/div><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[29],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts\/769"}],"collection":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/comments?post=769"}],"version-history":[{"count":3,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts\/769\/revisions"}],"predecessor-version":[{"id":774,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts\/769\/revisions\/774"}],"wp:attachment":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/media?parent=769"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/categories?post=769"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/tags?post=769"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}