{"id":647,"date":"2017-09-01T00:00:11","date_gmt":"2017-09-01T00:00:11","guid":{"rendered":"http:\/\/lbk.fe.uni-lj.si\/ic\/?p=647"},"modified":"2021-08-06T10:16:15","modified_gmt":"2021-08-06T10:16:15","slug":"system-for-dynamic-fluorescent-microscopy","status":"publish","type":"post","link":"https:\/\/lbk.fe.uni-lj.si\/ic\/en\/equipment\/system-for-dynamic-fluorescent-microscopy","title":{"rendered":"Motorised System for Dynamic Fluorescence Microscopy Leica Thunder"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: rgba(255,255,255,0);background-position: center center;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start\" style=\"max-width:1144px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_2_3 2_3 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-1\"><h3>Thunder Imager Live Cell system consists of fully motorized inverted fluorescent microscope DMi8 (Leica) with adaptive focus control for drift correction and fast focusing, deep-cooled 4.2 MP sCMOS Leica DFC9000 Gt fast camera, black incubator i8 BLACK LS (PeCon) with optional CO<sub>2<\/sub> and temperature control. Images are acquired with the software LAS X. As a light source, a system of 8 LED diodes, the LED8 (395, 438, 475, 511, 555, 575, 635, 730 nm) and UV light source the CoolLED pE340<sup>fura<\/sup> (CoolLED) are available.<\/h3>\n<p><u>Available are:<\/u><\/p>\n<ul>\n<li>Objectives:\n<ul>\n<li>N-plan 5x\/0.12 PH 0 (No. 11506303, Leica)<\/li>\n<li>HC PL FLUOTAR 10x\/0.32 PH 1 (No. 11506522, Leica)<\/li>\n<li>HC PL FLUOTAR 20x\/0.55 PH 2 (No. 11506518, Leica)<\/li>\n<li>HC PL FLUOTAR L 40x\/0.60 Corr. (No. 11506201, Leica)<\/li>\n<li>HC PL FL 63x\/0.90 Corr. (No. 11506223, Leica)<\/li>\n<li>HC FLUOTAR (340) 100x\/1.30, oil (No. 11506368, Leica)<\/li>\n<\/ul>\n<\/li>\n<li>Filters:\n<ul>\n<li>Filter cube DAPI (excitation: 350\/50, dichroic: 400, emission: 460\/50)<\/li>\n<li>Filter cube CYR71010 for use with multi-LED light sources (excitation: 436\/28, 506\/21, 578\/24, 730\/40; dichroic: 459, 523, 598, 763, emission: 473\/22, 539\/24, 641\/78, 810\/80)<\/li>\n<li>Filter cube DFT51010 for use with multi-LED light sources (excitation: 391\/32, 479\/33, 554\/24, 638\/31, dichroic: 415, 500, 572, 660, emission: 435\/30, 519\/25, 594\/32, 695\/58)<\/li>\n<li>Filter wheel LED8 with five filters (460\/80, 535\/79, 590\/50, 642\/80, 100%)\u200b<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-0{width:66.666666666667% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-column-0 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 2.88%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 2.88%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-0{width:100% !important;order : 0;}.fusion-builder-column-0 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-0{width:100% !important;order : 0;}.fusion-builder-column-0 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_3 1_3 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><style type=\"text\/css\">.fusion-gallery-1 .fusion-gallery-image {border:0px solid #e2e2e2;}<\/style><div class=\"fusion-gallery fusion-gallery-container fusion-grid-1 fusion-columns-total-2 fusion-gallery-layout-grid fusion-gallery-1\" style=\"margin:-5px;\"><div style=\"padding:5px;\" class=\"fusion-grid-column fusion-gallery-column fusion-gallery-column-1 hover-type-zoomin\"><div class=\"fusion-gallery-image\"><a href=\"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-content\/uploads\/2017\/09\/th1m_v2-1.jpg\" rel=\"noreferrer\" data-rel=\"iLightbox[gallery_image_1]\" class=\"fusion-lightbox\" target=\"_self\"><img src=\"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-content\/uploads\/2017\/09\/th1m_v2-1.jpg\" width=\"800\" height=\"533\" alt=\"\" title=\"th1m_v2\" aria-label=\"th1m_v2\" class=\"img-responsive wp-image-681\" \/><\/a><\/div><\/div><div class=\"clearfix\"><\/div><div style=\"padding:5px;\" class=\"fusion-grid-column fusion-gallery-column fusion-gallery-column-1 hover-type-zoomin\"><div class=\"fusion-gallery-image\"><a href=\"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-content\/uploads\/2017\/09\/th2m-1.jpg\" rel=\"noreferrer\" data-rel=\"iLightbox[gallery_image_1]\" class=\"fusion-lightbox\" target=\"_self\"><img src=\"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-content\/uploads\/2017\/09\/th2m-1.jpg\" width=\"800\" height=\"533\" alt=\"\" title=\"th2m\" aria-label=\"th2m\" class=\"img-responsive wp-image-659\" \/><\/a><\/div><\/div><div class=\"clearfix\"><\/div><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-1{width:33.333333333333% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-column-1 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 5.76%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 5.76%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-1{width:100% !important;order : 0;}.fusion-builder-column-1 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-1{width:100% !important;order : 0;}.fusion-builder-column-1 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-flex-container.fusion-builder-row-1{ padding-top : 0px;margin-top : 0px;padding-right : 0px;padding-bottom : 0px;margin-bottom : 0px;padding-left : 0px;}<\/style><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[29],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts\/647"}],"collection":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/comments?post=647"}],"version-history":[{"count":8,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts\/647\/revisions"}],"predecessor-version":[{"id":690,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts\/647\/revisions\/690"}],"wp:attachment":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/media?parent=647"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/categories?post=647"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/tags?post=647"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}