{"id":576,"date":"2018-03-16T11:20:03","date_gmt":"2018-03-16T11:20:03","guid":{"rendered":"http:\/\/lbk.fe.uni-lj.si\/ic\/?p=576"},"modified":"2018-03-16T11:20:03","modified_gmt":"2018-03-16T11:20:03","slug":"neon-invitrogen","status":"publish","type":"post","link":"https:\/\/lbk.fe.uni-lj.si\/ic\/en\/electroporator\/neon-invitrogen","title":{"rendered":"Neon [Invitrogen]"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: rgba(255,255,255,0);background-position: center center;background-repeat: no-repeat;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;margin-bottom: 0px;margin-top: 0px;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_2_3 2_3 fusion-two-third fusion-column-first\" style=\"width:66.666666666667%;width:calc(66.666666666667% - ( ( 4% ) * 0.66666666666667 ) );margin-right: 4%;margin-top:0px;margin-bottom:20px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-1\"><p>The Neon Transfection System is a pulse generator, able to deliver longer microsecond pulses used in gene electrotransfer treatments. The cell suspension is transferred to a special tip which contains one gold electrode. Tip is mounted on a special pipette which is inserted in the electroporation chamber which contains the other electrode. The gap between the electrodes is maximized while the surface area is minimized to reduce pH changes and heat generation. The generator comes with predefined protocols and electroporation buffers for efficient gene transfection. Special tips and electroporation chambers are packed separately and enable sterile environment. The parameters of electric pulses can be easily changed via the touchscreen.<\/p>\n<\/div><div class=\"fusion-aligncenter\"><style type=\"text\/css\">.fusion-button.button-1 {border-radius:2px;}<\/style><a class=\"fusion-button button-flat fusion-button-default-size button-default button-1 fusion-button-default-span fusion-button-default-type\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-content\/uploads\/2018\/03\/Invitrogen-Neon.pdf\"><span class=\"fusion-button-text\">Neon Manual<\/span><\/a><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_3 1_3 fusion-one-third fusion-column-last\" style=\"width:33.333333333333%;width:calc(33.333333333333% - ( ( 4% ) * 0.33333333333333 ) );margin-top:0px;margin-bottom:20px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><style type=\"text\/css\">.fusion-gallery-1 .fusion-gallery-image {border:0px solid #e2e2e2;}<\/style><div class=\"fusion-gallery fusion-gallery-container fusion-grid-1 fusion-columns-total-1 fusion-gallery-layout-grid fusion-gallery-1\" style=\"margin:-5px;\"><div style=\"padding:5px;\" class=\"fusion-grid-column fusion-gallery-column fusion-gallery-column-1 hover-type-zoomin\"><div class=\"fusion-gallery-image\"><a href=\"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-content\/uploads\/2018\/03\/OK4A7582.jpg\" rel=\"noreferrer\" data-rel=\"iLightbox[gallery_image_1]\" class=\"fusion-lightbox\"><img src=\"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-content\/uploads\/2018\/03\/OK4A7582.jpg\" width=\"5760\" height=\"3840\" alt=\"\" title=\"OK4A7582\" aria-label=\"OK4A7582\" class=\"img-responsive wp-image-577\" \/><\/a><\/div><\/div><div class=\"clearfix\"><\/div><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[43],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts\/576"}],"collection":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/comments?post=576"}],"version-history":[{"count":1,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts\/576\/revisions"}],"predecessor-version":[{"id":579,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/posts\/576\/revisions\/579"}],"wp:attachment":[{"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/media?parent=576"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/categories?post=576"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lbk.fe.uni-lj.si\/ic\/wp-json\/wp\/v2\/tags?post=576"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}