Equipment

/Equipment
Equipment 2017-09-08T13:07:42+00:00

System for Dynamic Fluorescence Microscopy

It consists of Axiovert 200 (Zeiss) fluorescent inverted microscope with camera VisiCam 1280 (Visitron) and CO2 chamber. It has MetaMorph and MetaFluor image acquisition software. It has tuneable lightsource Polychromatic illumination system (VisiChrome).

Available are:

  • Objectives:
    • A-plan 10x/0.25
    • LD Achroplan 20x/0.40 Corr
    • LD Achroplan 40x/0.60 Corr
    • EC Plan-Neofluar 63x/0.95 Corr
    • Plan-Apochromat 63x/1.4 Oil
    • Plan-Apochromat 100x/1.4 Oil Iris
  • Filters:
    • Anneps- Chroma 71006 (Ex. 440nm, 530nm;Em. D605/54m)
    • Fura-Chroma 71000 (Ex. 340nm, 380nm; Em. D510/40m)
    • TOTO-Chroma 41028 (Ex. 514nm; Em. D535/30m)
    • DAPI- Chroma 31000 (Ex. 360nm; Em. D510/40m)
    • TMRE-Chroma 31003 (Ex. 549nm; Em. D580/30m)
    • GFP-Chroma 31019 (Ex. 425nm; Em. D505/40m)

 

Zeiss Axiovert 200 Manual

Flow Cytometer

Attune NxT (Life technologies).

Available are:

  • Excitation lasers:
    • Blue (488 nm)
    • Violet (405 nm)
    • Red (637 nm)
  • Autosampler for 96 and 384 well plate measurements

 

Attune NxT Manual

Spectrofluorometer – microplate reader

Microplate reader Infinite M200 (Tecan) with temperature regulation (heating) can be used for measuring absorbance and fluorescence top and bottom as well as module for cuvettes. It has monochromatic module for excitation and emission.

Available is:

  • Absorbance: Ex. 230-1000 nm ; OD 0-3
  • Fluorescence: Ex. 230-850 nm, Em. 280-850

 

Infinite M200 Manual